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D4764

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

Synonyme(s) :

double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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0.25 UNIT
65,50 $
1 UNIT
163,00 $
5 UNITS
536,00 $

65,50 $

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Changer de vue
0.25 UNIT
65,50 $
1 UNIT
163,00 $
5 UNITS
536,00 $

About This Item

Numéro CAS:
91080-16-9
Numéro CE :
293-507-3
Numéro MDL:
MFCD00151698
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52

65,50 $

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Qualité

for molecular biology

Niveau de qualité

200

Forme

lyophilized powder

Poids mol.

>20 kb

Température de stockage

2-8°C

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Description générale

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Spécificité

42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay.[1] It was used as reference to determine the DNA content in cartilage tissue digest samples.[2][3]
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Caractéristiques et avantages

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Notes préparatoires

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000353056
0000322784
0000322783
0000295449
0000295448

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
K Bishayee et al.
Cell proliferation, 46(2), 153-163 (2013-03-21)
Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa
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Questions

  1. how much is 1 unit (i.e. in ug or mg)?

    1 answer

    1. One unit, based on the A260 value, is approximately 50 µg. The general conversion formula is 20 units per milligram. The 0.25 UN package size will contain approximately 12.5 µg, the 1 jUN package size will contain approximately 50 µg, and the 5UN package size will contain approximately 250 µg.

      For additional product information, please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/289/542/d4764dat.pdf

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