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D0180

Sigma-Aldrich

Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse

clone DDC-109, ascites fluid

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About This Item

Numéro MDL:
MFCD00162261
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

DDC-109, monoclonal

Poids mol.

antigen ~50 kDa

Contient

15 mM sodium azide

Espèces réactives

rabbit, sheep, guinea pig, monkey, rat, human, bovine, canine

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using bovine brain extract

Isotype

IgG2b

Numéro d'accès UniProt

P20711

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DDC(1644)
rat ... Ddc(24311)

Description générale

DDC is a 85-90 kDa molecular weight protein that is mainly expressed in brain, kidney and liver.
Monoclonal Anti-DOPA Decarboxylase (DDC) (mouse IgG2b isotype) is derived from the DDC-109 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse may also be used in immunoprecipitation, immunocytochemistry and ELISA applications. In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers. It is suitable for use in western blotting at a working dilution of 1:1000 using bovine brain extract. It was used for immunoblotting and immunoprecipitation of AADC (aromatic amino acid decarboxylase) from rat striatum in a study. It was used as a primary antibody for western blotting and co-immunoprecipitation studies using human embryonic kidney cells and human neuroblastoma cells.

Actions biochimiques/physiologiques

DOPA Decarboxylase (DDC), which requires pyridoxal phosphate for its activity, decarboxylates 5-hydroxytryptophan to form serotonin. It also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic neurons.
DOPA decarboxylase (DDC, EC 4.1.1.28), catalyzes the decarboxylation of the 5-hydroxytrptophan (5-HTP) to 5-HT. It requires pyridoxal phosphate for its activity. This is the enzyme that also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic.. Antibodies that react specifically with the enzyme enable the identification of both noradrenergic, dopaminergic, and serotoninergic systems, since DDC is present and active in all three types of neurons.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Eukaryotic-type aromatic amino acid decarboxylase from the root colonizer Pseudomonas putida is highly specific for 3, 4-dihydroxyphenyl-L-alanine, an allelochemical in the rhizosphere
Koyanagi T, et al.
Microbiology, 158(12), 2965-2974 (2012)
Roya Tehranian et al.
Journal of neurochemistry, 99(4), 1188-1196 (2006-09-20)
Alpha-synuclein is a presynaptic protein strongly implicated in Parkinson's disease (PD). Because dopamine neurons are invariably compromised during pathogenesis in PD, we have been exploring the functions of alpha-synuclein with particular relevance to dopaminergic neuronal cells. We previously discovered reduced
Anke A Dijkstra et al.
Cerebral cortex (New York, N.Y. : 1991), 28(1), 131-144 (2016-12-04)
The human anterior cingulate and frontoinsular cortices are distinguished by 2 unique Layer 5 neuronal morphotypes, the von Economo neurons (VENs) and fork cells, whose biological identity remains mysterious. Insights could impact research on diverse neuropsychiatric diseases to which these
C A O'Farrell et al.
Neuroscience, 164(3), 1127-1137 (2009-09-19)
A specific mutation (DeltaE302/303) in the torsinA gene underlies most cases of dominantly inherited early-onset torsion dystonia. This mutation causes the protein to aggregate and form intracellular inclusion bodies in cultured cells and animal models. Co-expression of the wildtype and

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