The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anionstatus in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.
Actions biochimiques/physiologiques
The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
Remarque sur l'analyse
The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.
Composants de kit seuls
Réf. du produit
Description
Assay Buffer 25 mL
Assay Medium 20 mL
Enhancer Solution, 40 mM 500 μL
Luminol Solution 500 μL
Xanthine Solution 500 μL
Xanthine Oxidase .5 U
Superoxide Dismutase from bovine liver, 4 units/μL 400 U
Code de la classe de stockage
10 - Combustible liquids
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