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C9301

Sigma-Aldrich

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

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About This Item

Numéro CAS:
9007-34-5
Numéro CE :
232-697-4
Numéro MDL:
MFCD00130825
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

product name

Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture

Source biologique

chicken (Sternal cartilage)

Niveau de qualité

300

Type

Type II (Miller)

Gamme de produits

BioReagent

Forme

powder

Conditionnement

glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg

Concentration

60-80% (biuret)

Technique(s)

cell culture | mammalian: suitable

Couverture de surface

6‑10 μg/cm2

Solubilité

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

Numéro d'accès UniProt

P02460

Spécificité de la liaison

Peptide Source: Fibrinogen

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

chicken ... COL2A1(395069)

Catégories apparentées

Description générale

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used:
  • in enzyme–linked immunosorbent assay (ELISA).
  • chondrocyte-mediated tissue production in vitro.
  • induction, treatment and assessment of collagenα induced arthritis (CIA).
  • cell proliferation assay.
  • as a coating for cell culture surfaces.

Actions biochimiques/physiologiques

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Caractéristiques et avantages

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Autres remarques

Major component of articular cartilage.

Notes préparatoires

Prepared by a modification of the method of Trentham, D.E., et al.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Cytokine profiles in the joint depend on pathology, but are different between synovial fluid, cartilage tissue and cultured chondrocytes.
Tsuchida A I, et al.
Arthritis Research & Therapy, 16(5), 441-441 (2014)
Mesenchymal-Epithelial Interactions in Neural Development (2013)
Biology of Proteoglycans (2013)
Shifeng Pan et al.
Experimental and therapeutic medicine, 23(2), 139-139 (2022-01-25)
Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes erosion of articular cartilage and bone and has adverse effects on both patients and livestock animals. The aim of the present study was to investigate the role of interleukin-1β (IL-1β)
Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.
Hoek R M, et al.
Arthritis and Rheumatism, 62(4), 1036-1042 (2010)
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