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C9210

Sigma-Aldrich

Cyanogen bromide-activated Agarose

lyophilized powder

Synonyme(s) :

Agarose, cyclic carbonimidate, CNBr-Activated Agarose

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1 G
150,00 $
5 G
496,00 $
15 G
1 280,00 $

150,00 $


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Devis pour commande en gros

Sélectionner une taille de conditionnement

Changer de vue
1 G
150,00 $
5 G
496,00 $
15 G
1 280,00 $

About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

150,00 $


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Devis pour commande en gros

Forme

lyophilized powder

Niveau de qualité

Technique(s)

affinity chromatography: suitable

Matrice

cross-linked 4% beaded agarose

Groupe de la matrice active

cyanate or related structures

Espaceur de matrice

1 atom (when ligands are coupled via isourea derivative or related linkage)

Gonflement

1 g swells to 2.5-4.5 mL

Capacité

≥10 mg/mL binding capacity (BSA)(via amino groups)

Adéquation

suitable for chromatography

Température de stockage

−20°C

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Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Forme physique

Lyophilized powder stabilized with lactose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of

Questions

1–4 of 4 Questions  
  1. What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?

    1 answer
    1. Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?

    1 answer
    1. The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.

      Helpful?

  4. What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?

    1 answer
    1. The control for this product is Product No. CL4B200 (Sepharose CL-4B).

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