C9080

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

clone V9, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Monoclonal Anti-Vimentin
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: CY3 conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: V9, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~58 kDa
• Espèces réactives: pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
• Technique(s): direct immunofluorescence: 1:200 using cultured cells; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil; immunohistochemistry (frozen sections): suitable using human tonsil
• Isotype: IgG1
• Numéro d'accès UniProt: P08670
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... VIM(7431) ; rat ... Vim(81818)

Description

Description générale

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.

Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

Spécificité

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Immunogène

pig eye lens vimentin.

Application

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:

• immunofluorescence and confocal microscopy
• cytoskeleton staining
• double labeling experiments
• immunohistochemical
• immunocytochemical localozation

Actions biochimiques/physiologiques

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).

Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Stockage et stabilité

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Informations légales

Cy is distributed under license from Amersham Biosciences Limited.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)