C5670
Calcium chloride
anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Synonyme(s) :
CaCl2, Calcium dichloride
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About This Item
Formule linéaire :
CaCl2
Numéro CAS:
Poids moléculaire :
110.98
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352207
ID de substance PubChem :
Nomenclature NACRES :
NA.71
Produits recommandés
Qualité
anhydrous
Pression de vapeur
0.01 mmHg ( 20 °C)
Gamme de produits
BioReagent
Pureté
≥96.0%
Forme
powder
Technique(s)
cell culture | insect: suitable
cell culture | plant: suitable
Pf
772 °C (lit.)
Chaîne SMILES
[Cl-].[Cl-].[Ca++]
InChI
1S/Ca.2ClH/h;2*1H/q+2;;/p-2
Clé InChI
UXVMQQNJUSDDNG-UHFFFAOYSA-L
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Description générale
Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10
Application
This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.
Notes préparatoires
This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
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