C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Synonyme(s) :
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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About This Item
Produits recommandés
Source biologique
Electrophorus electricus
Type
Type V-S
Forme
lyophilized powder
Activité spécifique
≥1,000 units/mg protein
Poids mol.
280 kDa
Composition
Protein, ≥45% biuret
pl
~5.3
Solubilité
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
Température de stockage
−20°C
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Description générale
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Application
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Actions biochimiques/physiologiques
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Attention
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Forme physique
Lyophilized powder containing Tris buffer salts
Remarque sur l'analyse
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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