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C-28020

Sigma-Aldrich

Hematopoietic Progenitor Medium

Ready-to-use kit including Basal Medium and SupplementMix, 100 ml

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About This Item

Code UNSPSC :
41161500

Conditionnement

pkg of 100 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Description générale

Hematopoietic Progenitor Medium (Ready-to-use)

Application

The Hematopoietic Progenitor Medium has been developed for the expansion of hematopoietic progenitors by means of differentiation into more committed lineages of blood cells. The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium, which contains all the necessary growth factors and supplements.

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Code de la classe de stockage

10 - Combustible liquids


Certificats d'analyse (COA)

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