The cell size is not a defined release criterion for this product. However, literature indicates that Human CD34+ Progenitor Cells typically have diameters ranging from 7 to 10 micrometers.
C-12921
Human CD34+ Progenitor Cells (hCD34+-CB)
Isolated from cord blood, single donor, 100,000 cryopreserved cells
Synonyme(s) :
hCD34+-CB cells
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1 EACH
1 800,00 $
1 800,00 $
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1 EACH
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
1 800,00 $
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Devis pour commande en gros
Produits recommandés
Source biologique
human umbilical cord blood
Conditionnement
pkg of 100,000 cells
Morphologie
(progenitor)
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
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Origine de la lignée cellulaire
Cord Blood
Application
CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.
Qualité
Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Avertissement
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Procédure de repiquage
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Autres remarques
Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive
Produits recommandés
Recommended Primary Cell Culture Media:Link
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Tatjana Weidner et al.
Nature protocols, 16(7), 3210-3240 (2021-04-14)
Receptor targeting of vector particles is a key technology to enable cell type-specific in vivo gene delivery. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them
Articles
CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.
Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.
Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.
Protocoles
In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
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Do you have any information about the size of these cells? I need a human cell that can be cultured but less than 5 micrometer. Thanks.
1 answer-
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Does it come from a single donor?
1 answer-
Yes, as stated following the product name, these cells are isolated from a single donor.
Helpful?
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I would like to know whether these are CD34+ progenitor cells isolated from cord blood and directly shipped after isolation or whether they are shipped after being cultured.
1 answer-
Yes, these cells are CD34+ progenitor cells isolated from cord blood. PromoCell Blood and Blood Progenitor Cells are cryopreserved directly after isolation (=P0). Customers will receive them cryopreserved. They haven’t been in culture before freezing.
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