The stability of this product in solution has not been evaluated. However, 172426 Glucoamylase (syn. Amyloglucosides) from Rhizopus, notes that solutions are stable when stored frozen in aliquots at -20°C for up to 3 months. Similar stability would be expected for A7420. Avoid freeze/thaw cycles of solutions.
A7420
Amyloglucosidase from Aspergillus niger
lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose
Synonyme(s) :
1,4-α-D-Glucane glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
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5 MG
81,60 $
25 MG
204,00 $
100 MG
610,00 $
81,60 $
Disponible pour expédition le09 avril 2025Détails
Sélectionner une taille de conditionnement
Changer de vue
5 MG
81,60 $
25 MG
204,00 $
100 MG
610,00 $
About This Item
81,60 $
Disponible pour expédition le09 avril 2025Détails
Produits recommandés
Forme
lyophilized powder
Activité spécifique
30-60 units/mg protein (biuret)
Composition
protein, ≥80%
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Impuretés
≤0.02% glucose
Autre catégorie plus écologique
, Enabling
Température de stockage
−20°C
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Catégories apparentées
Description générale
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [1].
Actions biochimiques/physiologiques
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[2] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[3][4]
Définition de l'unité
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
Forme physique
Lyophilized powder containing less than 0.02% glucose
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Protocoles
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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What is the shelf life of a solution (1 mg/mL in water) of Product A7420, Amyloglucosidase from Aspergillus niger and how should it be stored?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product A7420, Amyloglucosidase from Aspergillus niger?
1 answer-
Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
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Does Product A7420, Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
1 answer-
The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.
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How can a solution of Product A7420, Amyloglucosidase from Aspergillus niger, be prepared?
1 answer-
A 1 mg/mL solution may be prepared in water.
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