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A1602

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

Synonyme(s) :

1,4-α-D-Glucane glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

Numéro CAS:
9032-08-0
Numéro de classification (Commission des enzymes):
3.2.1.3 (BRENDA, IUBMB)
Numéro MDL:
MFCD00081350
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

ammonium sulfate suspension

Activité spécifique

≥40 units/mg protein

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Actions biochimiques/physiologiques

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Définition de l'unité

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Forme physique

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae
Teets NM, et al.
The Journal of Experimental Biology, 214(5), 806-814 (2011)
A method for routine measurements of total sugar and starch content in woody plant tissues
Chow PS and Landhausser SM
Tree physiology, 24(10), 1129-1136 (2004)
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries, 183-183 (2000)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores
Christina Pedersen et al.
PloS one, 13(3), e0194468-e0194468 (2018-03-21)
Insulin is a vital part of diabetes treatment, whereas glucagon is primarily used to treat insulin-induced hypoglycemia. However, glucagon is suggested to have a central role in the regulation of body weight, which would be beneficial for diabetic patients. Since
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocoles

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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