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A0802

Sigma-Aldrich

Adipic acid dihydrazide–Agarose

saline suspension

Synonyme(s) :

Adipic acid dihydrazide agarose beads

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About This Item

Numéro MDL:
MFCD00161954
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Source biologique

plant

Forme

saline suspension

Ampleur du marquage

≥8 μmol per mL

Technique(s)

microbe id | metabolite detection: suitable

Activation de la matrice

cyanogen bromide

Espaceur de matrice

11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)

Adéquation

suitable for chromatography

Température de stockage

2-8°C

Application

Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.

Forme physique

Suspension in 0.5 M NaCl containing preservative.

Mentions de danger

H412

Conseils de prudence

P273 - P501

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
M Caputi et al.
The EMBO journal, 18(14), 4060-4067 (1999-07-16)
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are
S C Datta et al.
The Journal of biological chemistry, 265(14), 8268-8274 (1990-05-15)
The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a
Alice Tianbu Zhang et al.
Journal of cell science, 124(Pt 12), 2058-2069 (2011-05-26)
Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of
Abrahem Abrahem et al.
Nucleic acids research, 36(16), 5201-5211 (2008-08-07)
Although RNA polymerases (RNAPs) are able to use RNA as template, it is unknown how they recognize RNA promoters. In this study, we used an RNA fragment derived from the hepatitis delta virus (HDV) genome as a model to investigate
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