The activity and protein concentration of this product varies from lot to lot and are reported in the product Certificate of Analysis. This information may then be used to determine the lot specific volume. As an example, a lot reporting a 99 mg/mL protein concentration would result in a volume of 2.53 mL for the 250 mg package size. This 250 mg package would have a total activity of 2436 units. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/76427#product-documentation
76427
Penicillin Amidase from Escherichia coli
5-10 units/mg protein
Synonyme(s) :
Penicillin Acylase, Penicillin Amidohydrolase
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250 MG
244,00 $
1 G
734,00 $
244,00 $
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250 MG
244,00 $
1 G
734,00 $
About This Item
244,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Source biologique
Escherichia coli
Niveau de qualité
Forme
suspension
Activité spécifique
5-10 units/mg protein
Poids mol.
Mr ~70000
Technique(s)
activity assay: suitable
Application(s)
diagnostic assay manufacturing
Température de stockage
2-8°C
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Description générale
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.[1]
Application
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.[2] It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.[3] Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.[4]
Actions biochimiques/physiologiques
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.[5] It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.[6]
Définition de l'unité
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
Yi-Han Lin et al.
Molecular microbiology, 47(3), 849-860 (2003-01-22)
N-acylhomoserine lactones (AHLs) are used as signal molecules by many quorum-sensing Proteobacteria. Diverse plant and animal pathogens use AHLs to regulate infection and virulence functions. These signals are subject to biological inactivation by AHL-lactonases and AHL-acylases. Previously, little was known
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
H J Duggleby et al.
Nature, 373(6511), 264-268 (1995-01-19)
Penicillin acylase (penicillin amidohydrolase, EC 3.5.1.11) is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. It is used on an industrial scale for the production of 6-aminopenicillanic acid, the starting material for the synthesis of semi-synthetic penicillins. Its
Lavinia Dunsmore et al.
Nature chemistry, 14(7), 754-765 (2022-06-29)
Natural products that contain ortho-quinones show great potential as anticancer agents but have been largely discarded from clinical development because their redox-cycling behaviour results in general systemic toxicity. Here we report conjugation of ortho-quinones to a carrier, which simultaneously masks
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Good afternoon, I recently received Penicillin-Amidase aus E. coli 5-10 units/mg protein (250mg). Could you please guide me how much volume does it contain so that we can calculate the precise amount of concenteration available. Thank you in advance.
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