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W1761

Millipore

Wilkins Chalgren anaerobic agar

suitable for microbiology, NutriSelect® Plus

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About This Item

Code UNSPSC :
41171605
Nomenclature NACRES :
NA.85

Stérilité

non-sterile

Niveau de qualité

200

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbiological culture: suitable

pH final

7.1±0.2 (25 °C)

Application(s)

cleaning products
clinical testing
cosmetics
food and beverages
personal care
microbiology

Adéquation

anaerobic bacteria
selective for Bacteroides spp.
selective for Bifidobacterium spp.
selective for Clostridium spp.

Catégories apparentées

Application

Used for the isolation of anaerobic bacteria.

Composants

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 5.00
Dextrose, 1.00
Sodium chloride, 5.00
L-Arginine, 1.00
Sodium pyruvate, 1.00
Hemin, 0.005
Menadione, 0.0005
Agar, 10.00

Notes préparatoires

Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates.For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement (Cat. No. N8781) or 2 vial of G.N. Spore Anaerobic Supplement (Cat. No. G3789) as desired into the sterile molten medium before pouring into sterile petri plates.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317,H412

Classification des risques

Aquatic Chronic 3 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificats d'analyse (COA)

Lot/Batch Number
BCCL7265
BCCJ2991
BCCG2225
BCCF1336
BCCC4351

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T D Wilkins et al.
Antimicrobial agents and chemotherapy, 10(6), 926-928 (1976-12-01)
A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%)

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