This product is a mixture of human serum albumin (HSA) and human gamma-globulin, the latter of which is essentially all human IgG. The E1% (at 280 nm) values for HSA and human IgG are 14 and 5.3, respectively. The lot-specific extinction coefficient for the mixture can be calculated using the protein concentrations provided the certificate of analysis (CofA)._x000D_
Sample calculation, for Lot SLCN8399:_x000D_
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8119-BULKSLCN8399.pdf_x000D_
The CofA reports an HSA concentration of 4.9 g/dL, and (by difference) a gamma-globulin concentration of 3.3 g/dL. The extinction coefficient at 280 nm for this 8.2 g/dL (82 mg/mL) lot is 86. Here is the calculation: (4.9 X 14) + (3.3 X 5.3) = 86.09, which would round to 86. If the standard were diluted by a factor of 8.2 to give a 1 g/dL or 1% solution, the E1% would be 10.5.
P8119
Protein Standard
analytical standard, 80 mg/mL (HSA and gamma-globulins)
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101,00 $
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About This Item
Code UNSPSC :
85151701
Nomenclature NACRES :
NA.24
101,00 $
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Devis pour commande en gros
Produits recommandés
Source biologique
human
Niveau de qualité
Qualité
analytical standard
Forme
liquid
Contient
0.05% sodium azide as preservative
Classe(s) chimique(s) de l'analyte
amino acids, peptides, proteins
Concentration
80 mg/mL (HSA and gamma-globulins)
Technique(s)
gel permeation chromatography (GPC): suitable
Application(s)
food and beverages
Format
multi-component solution
Température de stockage
2-8°C
Catégories apparentées
Application
Protein standard has been used in the determination of protein content of cod trypsin isoenzymes using bio-rad protein assay and ultraviolet-visible light spectrophotometry (UV-Vis).[1]
Autres remarques
Human serum albumin and gamma-globulins
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Lot/Batch Number
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What is the extinction coefficient for this 80 mg/mL protein standard?
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