11835289910

PCR DIG Labeling Mix^PLUS

sufficient for 2 x 50 assays (50μl final reaction volume), pkg of 2 × 250 μL, solution

• Synonyme(s) : DIG system, PCR labeling
• Code UNSPSC : 41105500

Propriétés

• Forme: solution
• Utilisation: sufficient for 2 x 50 assays (50μl final reaction volume)
• Conditionnement: pkg of 2 × 250 μL
• Fabricant/nom de marque: Roche
• Caractéristiques du produit alternatif plus écologique: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Autre catégorie plus écologique: , Aligned
• Température de stockage: −20°C

Description

Description générale

The PCR DIG Labeling Mix^PLUS is a mixture of lithium salts of deoxyadenosine triphosphate (dATP), deoxycytosine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), deoxyuridine triphosphate (dUTP) and digoxigenin (DIG)-11-dUTP. This nucleotide mixture can be added directly to polymerase chain reactions (PCR) and the DIG-labeled nucleotide will be incorporated into the PCR product. The incorporation of dUTP instead of deoxythymidine triphosphate (dTTP) allows the degradation of contaminating PCR products from previous amplification reactions with uracil-DNA glycosylase (UNG), preventing carryover contamination.The incorporated DIG label renders the sensitive detection of the PCR product with anti-DIG-conjugates, for example in the PCR ELISA, DIG-labling, and PCR ELISA, DIG-Detection. The PCR DIG Labeling Mix^PLUS gives comparable signal intensities to the PCR DIG Labeling Mix.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

PCR DIG Labeling MixPLUS is used for direct labeling of amplification products with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) in polymerase chain reactions (PCR) and for carryover prevention.The PCR DIG Labeling Mix^plus is 10x concentrated. For each reaction, add 10% of the final volume to the reaction mix.

Qualité

The PCR DIG Labeling Mix^PLUS is function tested.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash