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Principaux documents

10165948001

Roche

RNA, MS2

from bacteriophage MS2

Synonyme(s) :

RNA, ribonucleic acid

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About This Item

Code UNSPSC :
41106311

Source biologique

bacteriophage MS2

Niveau de qualité

Forme

solution

Poids mol.

~1200 kDa

Conditionnement

pkg of 500 μL (10 A260 units)

Fabricant/nom de marque

Roche

Technique(s)

RNA extraction: suitable

Couleur

colorless

Solubilité

water: miscible

Température de stockage

−20°C (−15°C to −25°C)

Catégories apparentées

Description générale

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Application

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Qualité

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Séquence

Chain Length 3,569 nucleotides

Notes préparatoires

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


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Consulter la Bibliothèque de documents

Monique I Andersson et al.
Wellcome open research, 5, 181-181 (2020-12-09)
Background: Laboratory diagnosis of SARS-CoV-2 infection (the cause of COVID-19) uses PCR to detect viral RNA (vRNA) in respiratory samples. SARS-CoV-2 RNA has also been detected in other sample types, but there is limited understanding of the clinical or laboratory
Yun Kun Ma et al.
Journal of virological methods, 157(2), 133-140 (2009-02-03)
PCR-based reverse transcriptase (RT) assays are highly sensitive for broad detection of retroviruses. These assays are currently used for demonstrating the absence of retroviral contamination in vaccines and can also be applied to clinical and laboratory research to investigate low-virus
Kirstine Kirkegaard et al.
Journal of assisted reproduction and genetics, 37(9), 2199-2209 (2020-07-19)
miRNAs have been suggested as biomarkers of embryo viability; however, findings from preliminary studies are divergent. Furthermore, the presence of other types of small RNA molecules remains to be investigated. The purpose of this study was to perform a comprehensive
Dennis M Timmerman et al.
Cancers, 13(17) (2021-09-11)
MicroRNAs (miRNAs) are short, non-coding RNAs involved in translation regulation. Dysregulation has been identified in cancer cells. miRNAs can be secreted and detectable in body fluids; therefore, they are potential liquid-biopsy biomarkers. The miR-371a-3 cluster members are an example, monitoring
Aitana Braza-Boïls et al.
International journal of molecular sciences, 21(8) (2020-04-25)
There is evidence for the effects of platelet inhibition on innate immune activation. Circulating microRNAs (miRNAs) have been implicated as markers of platelet and leukocyte activation. In the present study, we assessed the effects of P2Y12 inhibitors on platelet and

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