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R6750

Sigma-Aldrich

Ribonucleic acid from baker′s yeast (S. cerevisiae)

Synonyme(s) :

RNA

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About This Item

Numéro CAS:
63231-63-0
Numéro MDL:
MFCD00132195
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

Température de stockage

−20°C

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Description générale

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Application

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Notes préparatoires

Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
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Protocoles

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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