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10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

Synonyme(s) :

inorganic pyrophosphatase

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About This Item

Numéro de classification (Commission des enzymes):
Code UNSPSC :
12352204

Source biologique

yeast

Niveau de qualité

Forme

suspension

Activité spécifique

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

Conditionnement

pkg of 1 mL (1 mg)

Fabricant/nom de marque

Roche

Température de stockage

2-8°C

Description générale

Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

Spécificité

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Application

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification

Actions biochimiques/physiologiques

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

Qualité

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


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Consulter la Bibliothèque de documents

Michael A Kron et al.
Protein science : a publication of the Protein Society, 17(6), 983-989 (2008-04-16)
Diagnosis of eukaryotic parasitic infection using antibody-based tests such as ELISAs (enzyme-linked immunosorbent assays) is often problematic because of the need to differentiate between homologous host and pathogen proteins and to ensure that antibodies raised against a peptide will also
Tracking the m7G-cap during translation initiation by crosslinking methods.
Gross L, et al.
Methods, 137, 3-10 (2018)
2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.
Picard-Jean F, et al.
PLoS ONE, 13(3), e0193804-e0193804 (2018)
Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus.
Jespersen, et al.
Nature microbiology, 8, 1227-1239 (2023)
Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.
Kang Y, et al.
Nature Protocols, 10(7), 974-974 (2015)

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