MAB312R
Anti-A2B5 Antibody, clone A2B5-105
clone A2B5-105, Chemicon®, from mouse
Synonyme(s) :
Neuron Cell Surface Antigen
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
saturated ammonium sulfate (SAS) precipitated
Type de produit anticorps
primary antibodies
Clone
A2B5-105, monoclonal
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
Isotype
IgM
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Description générale
A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.
Spécificité
Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:
Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
Immunogène
Embryonic chicken retinal cells
Application
Anti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH.
Immunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Optimal working dilutions must be determined by end user.
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Forme physique
Ammonium Sulfate Precipitation
Format: Purified
Liquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA.
Stockage et stabilité
Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.
Remarque sur l'analyse
Control
Type II astrocytes, human neural progenitors
Type II astrocytes, human neural progenitors
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
M Fernandez et al.
The European journal of neuroscience, 20(8), 2059-2070 (2004-09-29)
Oligodendrocyte development and myelination are under thyroid hormone control. In this study we analysed the effects of chronic manipulation of thyroid status on the expression of a wide spectrum of oligodendrocyte precursor cells (OPCs) markers and myelin basic protein (MBP)
Peter Göttle et al.
Frontiers in cellular neuroscience, 15, 777542-777542 (2021-12-11)
Myelin repair in the adult central nervous system (CNS) is driven by successful differentiation of resident oligodendroglial precursor cells (OPCs) and thus constitutes a neurodegenerative process capable to compensate for functional deficits upon loss of oligodendrocytes and myelin sheaths as
Qingwei Lai et al.
Frontiers in molecular neuroscience, 10, 376-376 (2017-12-01)
Recently, it is reported that monocarboxylate transporter 1 (MCT1) plays crucial role in oligodendrocyte differentiation and myelination. We found that MCT1 is strongly expressed in oligodendrocyte but weakly expressed in oligodendrocyte precursors (OPCs), and the underlying mechanisms remain elusive. Histone
Nune Darbinian et al.
Neurobiology of disease, 148, 105181-105181 (2020-11-16)
Alterations of white matter integrity and subsequent white matter structural deficits are consistent findings in Fetal Alcohol Syndrome (FAS), but knowledge regarding the molecular mechanisms underlying these abnormalities is incomplete. Experimental rodent models of FAS have shown dysregulation of cytokine
Pascal Gervois et al.
Stem cells international, 2019, 8589149-8589149 (2019-05-16)
Pathologies of the central nervous system are characterized by loss of brain tissue and neuronal function which cannot be adequately restored by endogenous repair processes. This stresses the need for novel treatment options such as cell-based therapies that are able
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