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AP202

Sigma-Aldrich

Goat Anti-Rat light chain Antibody

Chemicon®, from goat

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

goat

Conjugué

unconjugated

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
western blot: suitable

Isotype

IgG

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Spécificité

Minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Immunogène

Epitope: Kappa light chain
Prepared from rat IgG light chain.

Application

Goat anti-Rat light chain Antibody is an antibody against light chain for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Western Blotting

ELISA

Optimal working dilutions must be determined by the end user.

Description de la cible

25 kDa

Forme physique

ImmunoAffinity Purified
Purified by immunoaffinity chromatography. Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6.

Stockage et stabilité

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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