ABN464

Anti-IRE1p Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Endoplasmic reticulum-to-nucleus signaling 1, Serine/threonine-protein kinase/endoribonuclease IRE1, ERN1, IRE1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès UniProt: O75460
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ERN1(2081)

Description

Description générale

Inositol-requiring protein 1 (IRE1p), also known as Ire1-alpha (IRE1a), Endoplasmic reticulum-to-nucleus signaling 1 (ERN1), and Serine/threonine-protein kinase/endoribonuclease IRE1, contains 1 KEN domain and 1 protein kinase domain.
IRE1p has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1p is ubiquitously expressed with high levels observed in pancreatic tissue.

Spécificité

This antibody recognizes the C-terminus of IRE1p.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IRE1p.

Application

Detect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.

Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected IRE1p in human pancreas and kidney tissues.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected IRE1p in A20 cells.

Immunofluorescence Analysis: 2 µg/mL from a representative lot detected IRE1p in A20 cells.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Qualité

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRE1p in A20 cell lysate.

Description de la cible

~114 kDa observed. Uncharacterized bands may appear in some lysate(s).

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
A20 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable