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AB5186

Sigma-Aldrich

Anti-Potassium Channel Kv2.1 Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KCNB1(3745)

Spécificité

Recognizes a full length Kv2.1 protein. Does not cross react with any other potassium channel antigens tested so far.

SPECIES REACTIVITIES: Should work in mouse (100% epitope homology) and in human and rabbit (88% epitope homology). Other species have not been tested.

Immunogène

Purified peptide from rat Kv2.1 (amino acids 841-857) (Accession P15387),

Application

Anti-Potassium Channel Kv2.1 Antibody detects level of Potassium Channel Kv2.1 & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1:250-1:500 using ECL on rat brain membranes.

Immunohistochemistry using rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Forme physique

Format: Purified
Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Stockage et stabilité

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 50 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Antagonistic roles of Ras-MAPK and Akt signaling in integrin-K+ channel complex-mediated cellular apoptosis.
Forzisi, et al.
Faseb Journal, 36, e22292-e22292 (2022)
Mark G MacAskill et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 62(4), 536-544 (2020-08-30)
Myocardial infarction (MI) is one of the leading causes of death worldwide, and inflammation is central to tissue response and patient outcomes. The 18-kDa translocator protein (TSPO) has been used in PET as an inflammatory biomarker. The aims of this
Vivian W Tang
Biology direct, 1, 37-37 (2006-12-13)
Zonula occludens, also known as the tight junction, is a specialized cell-cell interaction characterized by membrane "kisses" between epithelial cells. A cytoplasmic plaque of approximately 100 nm corresponding to a meshwork of densely packed proteins underlies the tight junction membrane
Dean O Smith et al.
PloS one, 3(2), e1604-e1604 (2008-02-14)
Because of the importance of voltage-activated K(+) channels during embryonic development and in cell proliferation, we present here the first description of these channels in E15 rat embryonic neural progenitor cells derived from the subventricular zone (SVZ). Activation, inactivation, and

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