431525

L-Leucine Dehydrogenase, Bacillus cereus

• Synonyme(s) : L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD ⁺-oxidoreductase
• Numéro CAS: 9082-71-7
• Numéro de classification (Commission des enzymes): 1.4.1.9 (BRENDA, IUBMB)
• Numéro MDL: MFCD00147461
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: Bacillus sp. (Bacillus cereus)
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥10 units/mg protein; ≥50 units/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD⁺-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.

Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.

Forme physique

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2