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CELLULYSIN® Cellulase, Trichoderma viride

Synonyme(s) :

β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54

Source biologique

fungus (Trichoderma viride)

Forme

solid

Activité spécifique

≥10,000 units/g dry wt.

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Technique(s)

transformation: suitable

Couleur

yellow-white

Solubilité

water: soluble

Adéquation

suitable for molecular biology

Application(s)

food and beverages
pharmaceutical

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Application

CELLULYSIN® Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
  • in the enzyme mix used for in situ hybridization to plant chromosomes
  • in the enzyme solution for the transformation of Phytophthora species
  • to isolate intact cells and protoplasts from plant tissues

Actions biochimiques/physiologiques

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Autres remarques

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing

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