17-10388
Di-Ubiquitin (Lys63-linked) Recombinant Protein
Native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.52
Produits recommandés
Niveau de qualité
Pureté
>95% (reversed phase HPLC)
Poids mol.
Mw ~17 kDa
Fabricant/nom de marque
Chemicon®
Conditions d'expédition
dry ice
Description générale
ANTIBODY AVAILABLE!
Anti-Ubiquitin, Lys63-Specific, clone Apu3 (catalogue # 05-1308)
This product is a native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).
This product is formed by chemical ligation.
Anti-Ubiquitin, Lys63-Specific, clone Apu3 (catalogue # 05-1308)
This product is a native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).
This product is formed by chemical ligation.
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
Application
LC-MS Analysis
Mobile phase A = 1% CH3CN, 0.1% formic acid in water (MilliQ) and B = 1% water (milliQ) and 0.1% formic acid in
CH3CN. Phenomenex Kinetex C18, (2.1×50 mm, 2.6 μM); flow rate = 0.6 mL/min, runtime = 6 min, column T = 40°C.
Gradient: 5% to 95% B over 3.5 min. (See Figure 2)
Mobile phase A = 1% CH3CN, 0.1% formic acid in water (MilliQ) and B = 1% water (milliQ) and 0.1% formic acid in
CH3CN. Phenomenex Kinetex C18, (2.1×50 mm, 2.6 μM); flow rate = 0.6 mL/min, runtime = 6 min, column T = 40°C.
Gradient: 5% to 95% B over 3.5 min. (See Figure 2)
Composants
50 µg of Di-Ubiquitin (Lys63-linked) reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM.
Qualité
Western Blot Analysis:
0.5-2 μg/mL of anti-Ubiquitin, Lys63-Specific, clone Apu2 (Cat. # 05-1308) detected Lys63-linked Ubiquitin from 1 µg of Di-Ubiquitin (Lys63-linked) Recombinant Protein (Cat. # 17-10388). (See Figure 1)
0.5-2 μg/mL of anti-Ubiquitin, Lys63-Specific, clone Apu2 (Cat. # 05-1308) detected Lys63-linked Ubiquitin from 1 µg of Di-Ubiquitin (Lys63-linked) Recombinant Protein (Cat. # 17-10388). (See Figure 1)
Stockage et stabilité
Store at -80°C. Avoid freeze/thaw cycles.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
188.6 °F - (refers to pure substance)
Point d'éclair (°C)
87 °C - (refers to pure substance)
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