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Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm × 2 mm HPLC Column

Synonyme(s) :

Chromolith® HighResolution RP-18 endcapped 100-2 HPLC column

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
SB.52

product name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 100 mm × 2 mm HPLC column

Niveau de qualité

Caractéristiques

endcapped

Ampleur du marquage

18% Carbon loading

Paramètres

200 bar pressure
50 °C max. temp.

Technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (High)

Colonne, L × D.I.

100 mm × 2 mm

Superficie

250 m2/g

Matrice

highly porous
monolithic silica

Groupe de la matrice active

C18 bonding phase, octadecylsilane (ODS)

Dimension de pores

1.5 μm macroporosity
15 nm mesoporosity

operating pH range

2-7.5

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Catégories apparentées

Description générale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), and the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for high-resolution separations meeting UHPLC and LC-MS needs.

Chromolith® HighResolution RP-18 endcapped columns with 2 mm internal diameter are ideal for use with UHPLC or UPLC instruments, thanks to their very small internal volumes. A particular benefit is very fast analysis, reduced sample preparation and the low column backpressure. Ultra-high performance and extremely low operating pressure make Chromolith® 2 mm I.D. columns truly unique.
Chromolith® and Chromolith® HighResolution columns are perfectly suitable for LC-MS use; however, the column dimension of 2 mm I.D. or below is preferred to be used in LC-MS methods.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52325] with the Chromolith® Guard Cartridge Holder 5 mm x 2 mm [1.52355]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Informations légales

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

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Articles

Development of an HPLC-UV method to determine Arbutin and Hydroquinone in whitening serum using a Chromolith® HighResolution C18 100x2mm HPLC column.

A comparative UHPLC analysis of diclofenac sodium in gel using Chromolith® HighResolution RP-18 endcapped 100 x 2 mm I.D column and FPP 1.7 µm hybrid silica C18 100 x 2.1 mm I.D column with UV and MS detection.

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

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