07-448-S

Anti-monomethyl-Histone H3 (Lys27) Antibody, Trial Size

Upstate^®, from rabbit

• Synonyme(s) : H3K27me1, Histone H3 (monomethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, vertebrates
• Fabricant/nom de marque: Upstate^®
• Technique(s): ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_003484
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: monomethylation (Lys27)
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spécificité

Broad species cross-reactivity expected, based on sequence homology.

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Immunogène

Epitope: Lys27

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Application

Anti-monomethyl-Histone H3 (Lys27), Trial Size Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 27 (H3K27me1). This antibody has dot blot (DB) verified specificity and is validated in, ICC, WB, ChIP, ChIP-seq. Small pack size is ideal for screening.

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualité

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Description de la cible

17 kDa

Forme physique

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 0.01 M phosphate buffer, pH 7.6, 0.125 M NaCl, 0.05% sodium azide and 30% glycerol.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa acid extract

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable