05-1106
Anti-IGF-IR Antibody, β-subunit, clone 1-2
clone 1-2, from mouse, purified by affinity chromatography
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
1-2, monoclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, rat
Réactivité de l'espèce (prédite par homologie)
human
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG2b
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... IGF1R(3480)
Description générale
IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.
Spécificité
Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.
Immunogène
A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.
Epitope: Between a.a. 1323-1337 of IGF-1R
Application
Anti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).
Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of IGF Monoclonal Antibody: Placenta
Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.
Optimal working dilutions must be determined by end user.
Optimal Staining of IGF Monoclonal Antibody: Placenta
Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Qualité
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.
Description de la cible
Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)
Liaison
Replaces: MAB1123
Forme physique
Affinity purified
Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Stockage et stabilité
Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Remarque sur l'analyse
Control
C2C12 Cell Lysate
C2C12 Cell Lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Yi Sui et al.
Stem cell research & therapy, 11(1), 86-86 (2020-02-28)
Parthenogenetic stem cells (PSCs) are a promising source of regenerated cardiomyocytes; however, their application may be limited without a paternal genome. Insulin-like growth factor-II (IGF-II), a paternally expressed growth hormone, is critical in embryonic differentiation. This study investigated whether forced
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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