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03-0196-00

Millipore

SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

Synonyme(s) :

Human α-synuclein, α-synuclein, Human total-α-synuclein, a-synuclein, total-a-synuclein

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Espèces réactives

canine, rat, feline, mouse

Fabricant/nom de marque

SMC®

assay range

sensitivity: 1.95  pg/mL
(LLOQ of Total α-synuclein)
inter-assay cv: 14%
intra-assay cv: 9%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days)

Technique(s)

single molecule counting: suitable

Description générale

The SMC® Human Total-α-Synuclein High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Spécificité

Species Reactivity Note

Specificity to the following species samples were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 100%
Beagle – 100%

Cross Reactivity

Cross-reactivity to the following analytes were tested with the following results:
Tau – 0%UCHL1 – 0%
β-synuclein – 0%
γ-synuclein – 0%

Accuracy

CSF Recovery: 5 CSF samples spiked with average recovery of 96% and a range of 90-102%
Serum Recovery: 4 serum samples spiked with average recovery of 96% and a range of 93-98%
Plasma Recovery: 4 plasma samples spiked with average recovery of 96% and a range of 93-97%

Application

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

Conditionnement

96 well assay

Composants

  • Assay Buffer
  • Total α-synuclein Coated Beads
  • Standard Diluent
  • Total α-synuclein Detection Antibody
  • Total α-synuclein Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Autres remarques

  • This new streamline kit has the following features:
  • Improved options for sample prep and incubation
  • Lyophilization protein standard for uniform shipping and storage condition
  • Increased reagent volumes to accommodate automation
  • Able to achieve assay specifications on both SMCxPRO® and Erenna.

Informations légales

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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