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SAB4200297

Sigma-Aldrich

Anti-Tensin 1 (C-terminal region) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-TNS, Anti-TNS1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 225 kDa (rat)
antigen ~150 kDa (Human)

species reactivity

rat, chicken, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 2-4 μg/mL using chicken embryo fibroblast cells
western blot: 1-2 μg/mL using HEK-293T cell lysates overexpressing human tensin1
western blot: 1.5-3.0 μg/mL using lysates of A10 cells

UniProt accession no.

Q9HBL0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNS1(7145)
rat ... Tns1(301509)

General description

Tensins are a new family of focal adhesion proteins that link between the extracellular matrix (ECM) and the cytoskeleton via integrins. The tensin family includes four members: tensin1-4, encoded by different genes. Tensins are multidomain proteins consisting of homologous C1, protein tyrosine phosphatase (PTPase), C2, SH2 and PTB domains. Tensin 1 (also known as TNS1), shares extensive homology with tensin 2 and tensin 3 at its N- and C-terminal regions. Tensin 1 mRNA is broadly expressed in mouse embryo and various human tissues and is localized to focal adhesions.

Immunogen

synthetic peptide corresponding to a sequence located near the C-terminus of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.

Application

Anti-Tensin 1 (C-terminal region) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Tensin 1 plays an important role in regulating cell polarization, shape, motility and invasion. Tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of tensin-promoted cell migration by its focal adhesion localization and the SH2 domain
Chen H and Lo SH
The Biochemical Journal, 370, 1039-1045 (2003)
Alexandra Woychek et al.
Cytoskeleton (Hoboken, N.J.), 76(2), 200-208 (2019-01-23)
The nuclear envelope protein nesprin-2G is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex and is responsible for mechanical and signaling crosstalk between the nucleus and cytoskeleton. A prior study has demonstrated that nesprin-2G knockout mice show
Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration
Chen H, et al.
Proceedings of the National Academy of Sciences of the USA, 99(2), 733-738 (2002)
Interaction of Axl receptor tyrosine kinase with C1-TEN, a novel C1 domain-containing protein with homology to tensin
Hafizi S, et al.
Biochemical and biophysical research communications, 299(5), 793-800 (2002)
Alexandra Woychek et al.
Cytoskeleton (Hoboken, N.J.), 76(2), 200-208 (2019-01-23)
The nuclear envelope protein nesprin-2G is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex and is responsible for mechanical and signaling crosstalk between the nucleus and cytoskeleton. A prior study has demonstrated that nesprin-2G knockout mice show

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