MAB3516
Anti-Heat Shock Protein 70 Antibody, clone W27
clone W27, Chemicon®, from mouse
Synonym(s):
HSP70
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
W27, monoclonal
species reactivity
bovine, human, monkey
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSPA4(3308)
Specificity
Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
Immunogen
HSP70 from HeLa cells.
Application
Immunoblotting: 0.5-1 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.
Physical form
Format: Purified
Analysis Note
Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mutations in UBQLN2 have been associated with rare cases of X-linked juvenile and adult forms of amyotrophic lateral sclerosis (ALS) and ALS linked to frontotemporal dementia (FTD). Here, we report 1 known (c.1489C>T, p.Pro497Ser, P497S) and 3 novel (c.1481C>T, p.Pro494Leu
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Cellular heterogeneity is a hallmark of human neuroblastoma tumors and cell lines. Within a single neuroblastoma are cells from distinct neural crest lineages whose relative abundance is significant for prognosis. We postulate that a self-renewing multipotent tumor stem cell, which
A Franchi et al.
Journal of cellular biochemistry, 121(4), 2877-2888 (2019-11-07)
Cells can communicate with other neighboring or distant cells through the secretion of extracellular vesicles (EV), composed of a lipid bilayer and bearing surface molecules that allow them to recognize target cells. In this way, EV induce signaling via different
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