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A redox signalling globin is essential for reproduction in Caenorhabditis elegans.

Nature communications (2015-12-02)
Sasha De Henau, Lesley Tilleman, Matthew Vangheel, Evi Luyckx, Stanislav Trashin, Martje Pauwels, Francesca Germani, Caroline Vlaeminck, Jacques R Vanfleteren, Wim Bert, Alessandra Pesce, Marco Nardini, Martino Bolognesi, Karolien De Wael, Luc Moens, Sylvia Dewilde, Bart P Braeckman
RESUMO

Moderate levels of reactive oxygen species (ROS) are now recognized as redox signalling molecules. However, thus far, only mitochondria and NADPH oxidases have been identified as cellular sources of ROS in signalling. Here we identify a globin (GLB-12) that produces superoxide, a type of ROS, which serves as an essential signal for reproduction in C. elegans. We find that GLB-12 has an important role in the regulation of multiple aspects in germline development, including germ cell apoptosis. We further describe how GLB-12 displays specific molecular, biochemical and structural properties that allow this globin to act as a superoxide generator. In addition, both an intra- and extracellular superoxide dismutase act as key partners of GLB-12 to create a transmembrane redox signal. Our results show that a globin can function as a driving factor in redox signalling, and how this signal is regulated at the subcellular level by multiple control layers.

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Sigma-Aldrich
Citocromo c, ≥95% (SDS-PAGE)
Sigma-Aldrich
Anti-IgG de coelho (molécula inteira)–peroxidase, affinity isolated antibody
Sigma-Aldrich
Myoglobin from equine heart, ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder