- Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting.
Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting.
Science advances (2021-11-25)
Xukun Lu, Yu Zhang, Lijuan Wang, Leyun Wang, Huili Wang, Qianhua Xu, Yunlong Xiang, Chaolei Chen, Feng Kong, Weikun Xia, Zili Lin, Sinan Ma, Ling Liu, Xiangguo Wang, Hemin Ni, Wei Li, Yong Guo, Wei Xie
PMID34818044
RESUMO
[Figure: see text].
MATERIAIS
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Sigma-Aldrich
Meio M2, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Sigma-Aldrich
Protease, powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
Sigma-Aldrich
Proteína LIF recombinante de camundongo ESGRO®, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.
Sigma-Aldrich
Tyrode′s Solution, Acidic, liquid, sterile-filtered, suitable for mouse embryo cell culture