G1006465

HPLC Analysis of Humira on BIOshell A400 Protein C4 Column

application for HPLC

Propriedades

• técnica(s): HPLC: suitable
• parâmetros de teste: column: BIOshell A400 Protein C4, 10 cm x 2.1 mm, 3.4 μm particles (66825-U); mobile phase: [A] 80:20, (water, 0.1% TFA): (acetonitrile, 0.1% TFA); [B] 50:50, (water, 0.1% TFA):(acetonitrile, 0.1% TFA).; gradient: 45 to 57% B in 12 min; flow rate: 0.2 mL/min; pressure: 2450 psi (for Competitor A), 1230 psi for BIOshell (169 bar (for Competitor A), 85 bar for BIOshell); column temp.: 75 °C; sample: 0.3 μL Humira
• adequação: application for HPLC
• aplicação(ões): life science and biopharma

Descrição

Nota de análise

Humira is a biotherapeutic used to treat rheumatoid arthritis (RA), ulcerative colitis (UC), and chronic plaque psoriasis (Ps). Analysis of the mAb′s monomors, aggregates, and fragments was performed on a BIOshell A400 Protein C4 column as well as a comparable column on the market. The BIOshell A400 Protein C4 column shows greater retentivity as well as greater resolution of the monomer from the aggregates and drug fragments. Peak recoveries were essential equivalent for the two columns.

Outras notas

mAb diluted in 20 mM Tricine/KOH, pH 7 to 1 g/L

Informações legais