52977-U
HybridSPE® DPX Tip
Hamilton, Tip volume 300 μL, box of 96 tips, bed weight 30 mg
Sinônimo(s):
HybridSPE® DPX SPE Tips
About This Item
Produtos recomendados
product name
HybridSPE® DPX Tip, weight 30 mg (bed), Hamilton, tip volume 300 μL
linha de produto
HybridSPE®
composição
HybridSPE® sorbent
embalagem
box of 96 tips
técnica(s)
solid phase extraction (SPE): suitable
volume da ponta
300 μL
peso
30 mg (bed)
Grupo ativo da matriz
Zirconia-based phase
aplicação(ões)
food and beverages
compatibilidade
Hamilton
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Categorias relacionadas
Descrição geral
In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.
The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.
We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.
What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 μL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 μL.
Guideline for 30 mg tips - plasma/serum sample: 30-100 μL; precipitating agent: 90-300 μL
Guideline for 50 mg tips - plasma/serum sample: 100-300 μL; precipitating agent: 300-900 μL
Características e benefícios
- Minimal elution solvent volumes
- Rapid extraction times (less than 3 minutes/sample)
- High extraction efficiencies
- Easy to perform extractions
- Lower costs
- Higher throughput
- Minimal training required
- Environmentally friendly
- Eliminates ion suppression effects often associated with phospholipids
- Tips offered to fit many formats: Tecan, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.
Informações legais
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Conteúdo relacionado
A extração em fase sólida (SPE) é uma técnica usada para preparos e purificações rápidas e seletivas da amostra antes das análises cromatográficas (p. ex., HPLC, GC, TLC). A SPE é usada para trocar matrizes de amostras, concentrar analitos e remover interferências e contaminantes para melhorar dados e proteger a coluna analítica.
Solid phase extraction purifies samples for chromatographic analysis, improving data quality and protecting analytical columns.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
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