SRP5355

MYT1, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinônimo(s): DKFZp547K1610, FLJ20093, PKMYT1
• Número CAS: 187414-13-7
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected Sf9 cells
• Ensaio: ≥70% (SDS-PAGE)
• forma: buffered aqueous glycerol solution
• peso molecular: ~93 kDa
• nº de adesão NCBI: NM_004203
• aplicação(ões): cell analysis
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PKMYT1(9088)

Descrição

Descrição geral

The PKMYT1 (protein kinase, membrane associated tyrosine/threonine 1) gene is differentially expressed between fetal liver and postnatal liver. This gene is mapped to human chromosome 16p13.3. It is also known to be localized to immature oocytes. MYT1 (myelin transcription factor 1) is a member of a family of neural specific, zinc finger-containing DNA-binding proteins. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn2+, but not other divalent cations.

Ações bioquímicas/fisiológicas

The PKMYT1 (protein kinase, membrane associated tyrosine/threonine 1) gene expression is essential for disease progression of colorectal cancer and hepatocellular carcinoma. The action of Cdc2 (cyclin-dependent kinase 2), an essential molecule required for the induction of mitosis, is regulated by PKMYT1. PKMYT1 binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system.

forma física

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Nota de preparo

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable