SRP5292

PIP5K1A, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinônimo(s): RP11-68I18.9
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected Sf9 cells
• linha de produto: PRECISIO^®
• Ensaio: ≥70% (SDS-PAGE)
• Formulário: buffered aqueous glycerol solution
• atividade específica: 947-1281 nmol/min·mg
• peso molecular: ~86 kDa
• nº de adesão NCBI: BC007833
• aplicação(ões): cell analysis
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PIP5K1A(8394)

Descrição

Descrição geral

PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through ′frizzled′ and ′dishevelled′, the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP production and enhances viral replication in primary lymphocytes.

forma física

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Nota de preparo

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Informações legais

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable