SRP2082

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• Código UNSPSC: 12352200
• NACRES: NA.26

Propriedades

• fonte biológica: human
• recombinante: expressed in insect cells
• Ensaio: ≥60% (SDS-PAGE)
• forma: frozen liquid
• embalagem: pkg of 5 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: 150 μg/mL
• cor: clear colorless
• nº de adesão NCBI: NM_024425
• nº de adesão UniProt: P19544
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... WT1(7490)

Descrição

Ações bioquímicas/fisiológicas

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable