SRP2032
Topo I (C651-765) (C terminal domain) human
recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
Sinônimo(s):
TOPI
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.26
fonte biológica
human
recombinante
expressed in insect cells
Ensaio
≥80% (SDS-PAGE)
forma
frozen liquid
peso molecular
~15.2 kDa
embalagem
pkg of 5 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
concentração
500 μg/mL
cor
clear colorless
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... TOP1(7150)
Ações bioquímicas/fisiológicas
Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.
forma física
Clear and colorless frozen liquid solution
Nota de preparo
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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M R Redinbo et al.
Science (New York, N.Y.), 279(5356), 1504-1513 (1998-03-21)
Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination. The crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted
DNA topoisomerase poisons as antitumor drugs.
L F Liu
Annual review of biochemistry, 58, 351-375 (1989-01-01)
J J Champoux
Annual review of biochemistry, 70, 369-413 (2001-06-08)
DNA topoisomerases solve the topological problems associated with DNA replication, transcription, recombination, and chromatin remodeling by introducing temporary single- or double-strand breaks in the DNA. In addition, these enzymes fine-tune the steady-state level of DNA supercoiling both to facilitate protein
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