SRP2020

TR, β 1 isoform human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Sinônimo(s): ERBA-beta, GRTH, NR1A2, THR1, THRB1
• Código UNSPSC: 12352202
• NACRES: NA.26

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥70% (SDS-PAGE)
• forma: frozen liquid
• peso molecular: ~53 kDa
• embalagem: pkg of 10 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: 500 μg/mL
• cor: clear colorless
• nº de adesão NCBI: NM_000461
• nº de adesão UniProt: P10828
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... THRB(7068)

Descrição

Ações bioquímicas/fisiológicas

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TR α and TR β isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR β gene generates two isoforms, TR β1 and TR β2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP).

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable