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Key Documents

COO/COA

SRP2007

TFIIF (RAP74 subunit) human

Name: TFIIF (RAP74 subunit) human
Brand: SIGMA

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sinônimo(s):

BTF4, RAP74, TF2F1

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About This Item

Código UNSPSC:

12352202

NACRES:

NA.26

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥80% (SDS-PAGE)

forma

frozen liquid

peso molecular

~59 kDa

embalagem

pkg of 10 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

concentração

200 μg/mL

técnica(s)

western blot: suitable

cor

clear colorless

nº de adesão NCBI

NM_002096

nº de adesão UniProt

P35269

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... GTF2F1(2962)

Ações bioquímicas/fisiológicas

The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits that form a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. In addition to its role in transcription initiation, TFIIF can increase the specificity and efficiency of RNA polymerase II transcription, and can especially increase the rate of transcription elongation.

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.

O Flores et al.

The Journal of biological chemistry, 264(15), 8913-8921 (1989-05-25)

The purification and characterization of transcription factor IIF (TFIIF), a factor required for transcription by the RNA polymerase II machinery, is described. TFIIF was isolated from the previously described IIE protein fraction. TFIIF enters into the transcription cycle via a

Isolation of three proteins that bind to mammalian RNA polymerase II.

M Sopta et al.

The Journal of biological chemistry, 260(18), 10353-10360 (1985-08-25)

We have used affinity chromatography on columns containing immobilized calf thymus RNA polymerase II to isolate three phosphoproteins (RAP72, RAP38, and RAP30) that bind directly to RNA polymerase II. All could be isolated from cell nuclei, and all three could

Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.