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SRP2004

TFIIE α, p56 human

Name: TFIIE α, p56 human
Brand: SIGMA

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sinônimo(s):

FE, TF2E1, TFIIE-A

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About This Item

Código UNSPSC:

12352202

NACRES:

NA.26

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥70% (SDS-PAGE)

forma

frozen liquid

peso molecular

~51.5 kDa

embalagem

pkg of 10 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

concentração

200 μg/mL

técnica(s)

western blot: suitable

cor

clear colorless

nº de adesão NCBI

NM_005513

nº de adesão UniProt

P29083

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... GTF2E1(2960)

Ações bioquímicas/fisiológicas

The human Transcription Factor IIE (TFIIE) is composed of 56 kDa ( α) and 34 kDa ( β) subunits and is shown to be a heterotetramer. The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF.

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE.

J Inostroza et al.

The Journal of biological chemistry, 266(14), 9304-9308 (1991-05-15)

Mammalian RNA polymerase II transcription factor IIE (TFIIE) was purified to apparent homogeneity. The activity copurified with polypeptides of 34 and 56 kDa. The 56-kDa subunit was sufficient for low levels of transcription activity in a transcription system reconstituted in

Factors involved in specific transcription by mammalian RNA polymerase II: purification and characterization of general transcription factor TFIIE.

Y Ohkuma et al.

Proceedings of the National Academy of Sciences of the United States of America, 87(23), 9163-9167 (1990-12-01)

Human transcription factor TFIIE, a ubiquitous factor required for transcription initiation by RNA polymerase II, was purified to homogeneity by a combination of conventional and HPLC steps. The purified TFIIE contained equimolar amounts of 57-kDa (TFIIE-alpha) and 34-kDa (TFIIE-beta) polypeptides

Structure and functional properties of human general transcription factor IIE.

M G Peterson et al.

Nature, 354(6352), 369-373 (1991-12-05)

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex. We have isolated human complementary DNA clones for both the subunits of TFIIE. Using purified recombinant proteins we find that both subunits

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