SAB5801039

Goat Anti-Mouse kappa HRP Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinônimo(s): Recombinant Alpaca single variable domain secondary Nanobody
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: alpaca
• recombinante: expressed in HEK 293 cells (Human cell line)
• conjugado: horse radish peroxidase (HRP)
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: secondary antibodies
• clone: recombinant monoclonal
• Formulário: lyophilized solid
• peso molecular: 100 kDa
• reatividade de espécies: mouse, Alpaca
• técnica(s): ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• tamanho: 0.1 mL
• cor: colorless
• Isotipo: VHH
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Goat Anti-Mouse kappa HRP Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgG kappa chain. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG kappa chain coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Mouse kappa HRP Alpaca Heavy chain only Antibody reacts with the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Especificidade

Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc

Imunogênio

Recombinant Alpaca single variable domain secondary nano antibody

Embalagem

100 μg in Glass bottle

forma física

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstituição

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Armazenamento e estabilidade

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Outras notas

No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317,H412
• Declarações de precaução: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classificações de perigo: Aquatic Chronic 3 - Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable