SAB5801015

Alpaca Anti-Mouse kappa single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinônimo(s): Recombinant Alpaca single variable domain secondary Nanobody
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: alpaca
• recombinante: expressed in E. coli
• conjugado: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: secondary antibodies
• clone: recombinant monoclonal
• forma: lyophilized solid
• peso molecular: 14 kDa
• reatividade de espécies: mouse, Alpaca
• técnica(s): ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• tamanho: 0.1 mL
• cor: colorless
• Isotipo: VHH
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Alpaca Anti-Mouse kappa single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa single domain Antibody reacts with the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Especificidade

Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c

Imunogênio

Recombinant Alpaca single variable domain secondary nano antibody

Embalagem

100 μg in Glass bottle

forma física

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstituição

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Armazenamento e estabilidade

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Outras notas

No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317,H412
• Declarações de precaução: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classificações de perigo: Aquatic Chronic 3 - Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable