SAB4702131

Monoclonal Anti-CD22 FITC produced in mouse

• Sinônimo(s): SIGLEC-2, SIGLEC2
• Código UNSPSC: 12352203

Propriedades

• conjugado: FITC conjugate
• Nível de qualidade: 100
• tipo de produto de anticorpo: primary antibodies
• clone: S-HCL-1, monoclonal
• forma: buffered aqueous solution
• reatividade de espécies: human
• técnica(s): flow cytometry: suitable
• Isotipo: IgG2bκ
• nº de adesão UniProt: P20273
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CD22(933)

Descrição

Descrição geral

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Especificidade

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Imunogênio

Whole hairy cell leukemia cells and membrane preparation

forma física

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Nota de preparo

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Armazenamento e estabilidade

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Outras notas

The antibody solution should be gently mixed before use.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable