SAB4702058

Monoclonal Anti-CD45 APC-Cyanine7 produced in mouse

• Sinônimo(s): B220, GP180, LCA, LY5, T200, TPC
• Código UNSPSC: 12352203

Propriedades

• conjugado: APC-Cy7 conjugate
• Nível de qualidade: 100
• tipo de produto de anticorpo: primary antibodies
• clone: MEM-28, monoclonal
• forma: buffered aqueous solution
• reatividade de espécies: human
• técnica(s): flow cytometry: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P08575
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PTPRC(5788)

Descrição

Descrição geral

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Especificidade

The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Imunogênio

Human thymocytes and T lymphocytes.

forma física

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Nota de preparo

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Armazenamento e estabilidade

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Outras notas

The antibody solution should be gently mixed before use.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable