SAB4200210

Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Sinônimo(s): Anti-CPLA2-alpha, Anti-Group IVA, Anti-PLA2G4A PLA2G4, Anti-cytosolic phospholipase A2
• Número MDL: MFCD02686076
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~100 kDa
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• concentração: ~1.5 mg/mL
• técnica(s): western blot: 2-4 μg/mL using extracts of HEK-293T cells over expressing human cPLA₂
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... cPLA2(5321)

Descrição

Descrição geral

Cytosolic phospholipase A2, group IVa, (cPLA2, also known as cPLA2a, PLA2G4A) is a member of the PLA2 superfamily that catalyses the cleavage of fatty acids from the sn-2 position of phospholipids. PLA2 isoenzymes vary in their cellular localizations, Ca²⁺ sensitivities and substrate specificities. cPLA2 mRNA is widely expressed in tissues and in various cell types including platelets, macrophages and endothelial cells.

Aplicação

Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit has been used in immunoblotting.

Ações bioquímicas/fisiológicas

Cytosolic phospholipase A2 (cPLA2) catalyse the synthesis of precursors of proinflammatory mediators, such as prostaglandins and leukotrienes, through the release of arachidonic acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including intracellular membrane trafficking, differentiation, proliferation and apoptosis. They are thought to play a role in oxidative and inflammatory responses in cerebral ischemia, Alzheimer′s disease (AD) and neuronal injury. Upon cell stimulation, cPLA2 is activated by increased intracellular Ca²⁺ levels and phosphorylation, resulting in its translocation from the cytosol to the endoplasmic reticulum and the nuclear membrane. cPLA2 is phosphorylated and activated by either ERK1/2, p38 MAPK or JNK at three sites, Ser⁵⁰⁵, Ser⁵¹⁵ and Ser⁷²⁷, depending on the cell type and agonist.

Ligação

synthetic peptide corresponding to amino acids 242-258 of human cPLA2 conjugated to KLH. The corresponding sequence is identical in mouse and rat cPLA2

forma física

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable