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R5405

Sigma-Aldrich

Anti-Rabex-5 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-RAP1, Anti-Rab5 GDP/GTP exchange factor (RABGEF1), Anti-Rabaptin-5-associated exchange factor for Rab5

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~58 kDa

reatividade de espécies

mouse, rat, human

concentração

~1.0 mg/mL

técnica(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extracts of mouse brain and human U87 cells

nº de adesão UniProt

Q9UJ41

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... RABGEF1(27342)
mouse ... Rabgef1(56715)
rat ... Rabgef1(360797)

Descrição geral

Anti-Rabex-5 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at N-terminal of human Rabex-5, with an added cysteine, conjugated to KLH. Rabex-5 contains multiple functional domains, including a vacuolar protein sorting-associated (VPS9) domain that possesses the GDP/GTP exchange factors (GEF) activity, a ubiquitin-binding A20-like zinc finger (ZnF) domain, and a coiled-coil domain.

Aplicação

Anti-Rabex-5 (N-terminal) antibody may be used in immunoblotting and immunofluorescence.

Ações bioquímicas/fisiológicas

Rabex-5 forms a tight complex with Rabaptin-5 that is essential for endocytic membrane fusion. The bound Rabaptin-5 to Rabex-5 increases the GEF activity of Rabex-5 and fastens the generation of the active GTP-bound form of membrane associated Rab5.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 15(11), 1206-1218 (2014-08-02)
Listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell RAB5 GTPases. Listeria monocytogenes must subsequently inhibit RAB5, however, in order to escape lysosome-mediated destruction. Relatively
Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex
Lippe R, et al.
Molecular Biology of the Cell, 12(7), 2219-2228 (2001)
The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain
Mattera R, et al.
Test, 281(10), 6874-6883 (2006)
The RAB5-GEF Function of RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection.
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 16(7), 796-796 (2015-06-09)
David Millrine et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), 10625-10630 (2016-09-08)
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with

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